Journal: iScience

Article Title: IL-30 protects against sepsis-induced myocardial dysfunction by inhibiting pro-inflammatory macrophage polarization and pyroptosis

doi: 10.1016/j.isci.2023.107544

Figure Lengend Snippet: IL-30 knockout aggravates sepsis-induced inflammatory responses in the heart by promoting proinflammatory macrophage polarization and pyroptosis (A–E) The cardiac mRNA levels of IL-1β, IL-6, TNF-α, MCP-1 and CXCL2 in each group (n = 8). (F) Representative flow cytometry images and corresponding quantification showing the ratio of CD11B + F4/80 + macrophages in each group (n = 6). (G) Representative flow cytometry images and corresponding quantification showing the ratio of F4/80 + Ly6C high macrophages in each group (n = 6). (H) mRNA levels of CD80, CD86 and INOS in each group (n = 8). (I) mRNA levels of CD163 and CD206 in each group (n = 8). (J) Representative immunofluorescence staining images for F4/80 and caspase1 (n = 4). (K) Representative immunoblots and corresponding quantification showing cardiac tissue levels of NLRP3, IL-1β, IL-18 and GSDMD-nt (n = 4). Values represent the mean ± SEM. ∗ indicates p < 0.05, ∗∗ indicates p < 0.01, ∗∗∗ indicates p < 0.001. IL, interleukin; MCP-1, monocyte chemoattractant protein-1; CXCL2, C-X-C motif chemokine 2; INOS, inducible nitric oxide synthase; NLRP3, NOD-like receptor thermal protein domain associated protein 3; caspase 1, cysteinyl aspartate specific proteinase-1; GSDMD-nt, N-terminal gasdermin domain-containing protein 1.

Article Snippet: Caspase1 , Santa Cruz , Cat#SC-392736.

Techniques: Knock-Out, Flow Cytometry, Immunofluorescence, Staining, Western Blot

Journal: iScience

Article Title: IL-30 protects against sepsis-induced myocardial dysfunction by inhibiting pro-inflammatory macrophage polarization and pyroptosis

doi: 10.1016/j.isci.2023.107544

Figure Lengend Snippet: IL-30 deficiency promoted LPS-induced proinflammatory macrophage polarization and pyroptosis in vitro (A) Representative flow cytometry images and corresponding quantification showing the ratio Ly6C high macrophages in each group (n = 4). (B–D) mRNA level of IL-6, TNF-α, CD80 and CD86 (n = 4). (E) Representative immunoblots and corresponding quantification showing levels of NLRP3, caspase1, IL-1β and GSDMD-nt (n = 4). Values represent the mean ± SEM. ∗ indicates p < 0.05, ∗∗ indicates p < 0.01, ∗∗∗ indicates p < 0.001. IL, interleukin; NLRP3, NOD-like receptor thermal protein domain associated protein 3; caspase 1, cysteinyl aspartate specific proteinase-1; GSDMD-nt, N-terminal gasdermin domain-containing protein 1.

Article Snippet: Caspase1 , Santa Cruz , Cat#SC-392736.

Techniques: In Vitro, Flow Cytometry, Western Blot

Journal: iScience

Article Title: IL-30 protects against sepsis-induced myocardial dysfunction by inhibiting pro-inflammatory macrophage polarization and pyroptosis

doi: 10.1016/j.isci.2023.107544

Figure Lengend Snippet: Inhibiting NLRP3 with MCC950 blocked proinflammatory macrophage polarization and pyroptosis induced by IL-30 deficiency (A and B) LVEF and FS (n = 6). (C) Cardiac CK-MB (n = 6). (D) mRNA level of IL-6 and TNF-α (n = 8). (E) Representative flow cytometry images and corresponding quantification showing the ratio of F4/80 + Ly6C high macrophages in each group (n = 6). (F) Representative immunoblots and corresponding quantification showing cardiac tissue levels of caspase1, IL-1β and GSDMD-nt (n = 4). (G) Representative flow cytometry images and corresponding quantification showing the ratio of Ly6C high macrophages in each group (n = 4). (H) mRNA level of IL-6 and INOS (n = 4). (I) Representative immunoblots and corresponding quantification showing levels of caspase1, IL-1β and GSDMD-nt (n = 4). Values represent the mean ± SEM. ∗ indicates p < 0.05, ∗∗ indicates p < 0.01, ∗∗∗ indicates p < 0.001. IL, interleukin; CK-MB, creatine kinase-myocardial band; caspase 1, cysteinyl aspartate specific proteinase-1; GSDMD-nt, N-terminal gasdermin domain-containing protein 1.

Article Snippet: Caspase1 , Santa Cruz , Cat#SC-392736.

Techniques: Flow Cytometry, Western Blot

Journal: iScience

Article Title: IL-30 protects against sepsis-induced myocardial dysfunction by inhibiting pro-inflammatory macrophage polarization and pyroptosis

doi: 10.1016/j.isci.2023.107544

Figure Lengend Snippet: Recombinant IL-30 attenuates sepsis-induced cardiac dysfunction and injury by inhibiting proinflammatory macrophage polarization and pyroptosis (A) Survival rate (n = 10). (B) Cardiac function (n = 6). (C–F) The cardiac and serum levels of CK-MB and LDH were measured in each group (n = 6). (G–J) mRNA level of IL-6, TNF-α, MCP-1 and CXCL2 (n = 8). (K) Representative flow cytometry images and corresponding quantification showing the ratio of F4/80 + Ly6C high macrophages in each group (n = 4). (L) mRNA levels of CD80, CD86 and INOS in each group (n = 8). (M) mRNA levels of CD163 and CD206 in each group (n = 8). (N) Representative immunoblots and corresponding quantification showing levels of NLRP3, caspase1, IL-1β and IL-18 (n = 4). Values represent the mean ± SEM. ∗ indicates p < 0.05, ∗∗ indicates p < 0.01, ∗∗∗ indicates p < 0.001. IL, interleukin; CK-MB, creatine kinase-myocardial band; LDH, lactate dehydrogenase; MCP-1, monocyte chemoattractant protein-1; CXCL2, C-X-C motif chemokine 2; INOS, inducible nitric oxide synthase; NLRP3, NOD-like receptor thermal protein domain associated protein 3; caspase 1, cysteinyl aspartate specific proteinase-1.

Article Snippet: Caspase1 , Santa Cruz , Cat#SC-392736.

Techniques: Recombinant, Flow Cytometry, Western Blot

Journal: iScience

Article Title: IL-30 protects against sepsis-induced myocardial dysfunction by inhibiting pro-inflammatory macrophage polarization and pyroptosis

doi: 10.1016/j.isci.2023.107544

Figure Lengend Snippet: IL-30 inhibited proinflammatory macrophage polarization and pyroptosis in vitro (A) Representative flow cytometry images and corresponding quantification showing the ratio of Ly6C high macrophages in each group (n = 4). (B) mRNA levels of IL-6 and INOS (n = 4). (C) Representative immunoblots and corresponding quantification showing levels of NLRP3, caspase1, IL-1β and IL-18 (n = 4). (D) Representative immunoblots and corresponding quantification showing levels of JAK1, JAK2, STAT1, STAT3, P38, ERK, JNK and P65 (n = 6). Values represent the mean ± SEM. ∗ indicates p < 0.05, ∗∗ indicates p < 0.01, ∗∗∗ indicates p < 0.001, ns indicates no significance.

Article Snippet: Caspase1 , Santa Cruz , Cat#SC-392736.

Techniques: In Vitro, Flow Cytometry, Western Blot

Journal: iScience

Article Title: IL-30 protects against sepsis-induced myocardial dysfunction by inhibiting pro-inflammatory macrophage polarization and pyroptosis

doi: 10.1016/j.isci.2023.107544

Figure Lengend Snippet:

Article Snippet: Caspase1 , Santa Cruz , Cat#SC-392736.

Techniques: Staining, Recombinant, Liposomes, Bicinchoninic Acid Protein Assay, AST Assay, Lactate Dehydrogenase Assay, cDNA Synthesis, Enzyme-linked Immunosorbent Assay, Knock-Out, Software